Like Mike Koleosho already mentioned, my document on http://scn.sap.com/docs/DOC-63388 helps you with a rough estimation. Also keep in mind that you take an additional 10% of extra work into account for defects, incidents etc.
Offcourse the planning should be based on a DTAP like environment.
Yes this can affect the estimation because you need to transfer/transport through all the environments.The quickest solution for transport is with the use of ATL file loading.
Hopefully this will help you. Good luck with your estimation!
Bluesky
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