Hello Mahesh
Why do you want to backup to E drive first and then copy to F drive? You can straight away copy to F drive or where ever you want.
Just login into OS level as db2<sid>, open command prompt and run the below command.
db2 backup ...
After loosing all data in ec2, we redeployed DEV & QA on EC2. The key is schedule your back ups on time and have multipe virtual images, just in case one image has an issue. you should be fine.Best RegardsSmr